Are your PAP smear slides fixed in alcohol? If so, you probably don't need antigen retrieval.
Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "hao zhang" <[EMAIL PROTECTED]> 09/13/08 11:11 PM >>> Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet