Dorothy, We've done several comparisons like this for amyloid staining. In our experience, 8um works fine. We did see stronger staining (birefringence) in some thicker sections with Congo Red but I don't think there would be a significant improvement in staining (sensitivity?) from 8um to 10um. I wouldn't cut sections any thinner than 8um but you can still get amyloid staining at 4um. It would be relatively easy to take your controls and cut them different thicknesses just to prove the point. Good luck!
Mark A Burton HTL ASCP Lab Manager & Sr. Histotechnologist Molecular Aging & Development Lab Boston University Medical Campus -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Webb, Dorothy L Sent: Tuesday, September 16, 2008 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
