Greetings all,
I'm wondering whether there are any recommendations for how to effectively cryosection dorsal mouse skin for immunohistochemistry. I'm having difficulty with much of the mouse skin slipping off the slide during the fixation and washing steps, which seems to result in loss of the epidermis in particular, that part of the tissue that I happen to be interested in. I apply the dorsal mouse skin cryosections onto SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I use acetone for fixation, 10 minutes, and use PBS for washing. Any suggestions? This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
