Yes, the immediate effect of acetic is nuclear swelling. René J. --- On Tue, 9/23/08, Trajkovic, Dusko <[EMAIL PROTECTED]> wrote:
From: Trajkovic, Dusko <[EMAIL PROTECTED]> Subject: [Histonet] Effect of 5%Aeti acid on Nuclei To: Histonet@lists.utsouthwestern.edu Date: Tuesday, September 23, 2008, 9:58 AM Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet