Angela, we use NBF for at least 16 hours, the next day after receiving they are put in decal (mixture of formic acid and hydrochloric acid with formaldehyd) for 7-8 hours. Our biopsies are about 3-4 mm thick. After acid decalcification enzyme-stains are not possible any more and the iron-stain could be false negative. But we have good experiences with the IHC and had even an improvement with some markers compared with EDTA-decal.
Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Ursprüngliche Nachricht----- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Angela R. Murray Gesendet: Mittwoch, 24. September 2008 19:40 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone Marrow Biopsy Fixation Have you fixed bone marrow biopsies in formaling and if so for how long and what decal solution is best? -------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet