I recently started paraffin sectioning and I am having trouble with getting
good quality sections reproducibly. I was wondering if you have any tips that
may help. I am sectioning thymus, pancreas, and lung. Section thickness is 10
um. The microtome that I am using is Reichert-Jung Supercut, disposable knife
holder by Leica, and Accu-Edge low profile blades. I fix the tissue in a
modified Carnoy's fix overnight, dehydrate in graded alcohol, 100% ETOH,
Toluene 2x15 minutes, Paraffin at 65 degrees overnight with 2X changes of
paraffin. Embed and store at RT. The paraffin I am using is Fisherbrand
Paraplast X-tra Tissue embedding medium. Any suggestion would be greatly
appreciated.
Thank you in advance,
James Dooley
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