Sarah: Histoclear, also known as Histolene is a d-Limonene based xylene substitute and there are 13 opinions about it in HistoNet that I will try to summarize for you: 1-not as good as Xylene to dewax before IHC 2-has a strong odor 3-hardens brain, liver and spleen 4-not good for cover slipping 5-incompatible with xylene or toluene based cover medium 6- has produced skin rashes 7- has produced headache problems 8- fades hematoxylin 9-avoid Strong oxidizer when using it 10- has to be used with Histomount or Omnimount medium 11- generally speaking is a poor dewaxing agent 12- one posting concluded as "avoid it" On the other hand Wynnchuk (1994) says is as good as xylene, but Faolain et al (2005) wrote that it does not dewax well. I am sure that there will be some that "love it", but I personally would not use it based on the above. Besides that it costs $59.29/gal or 1.47 times as much as xylene. René J.
--- On Wed, 10/1/08, Hale, Sarah <[EMAIL PROTECTED]> wrote: From: Hale, Sarah <[EMAIL PROTECTED]> Subject: [Histonet] Histoclear vs. Xylene To: [email protected] Date: Wednesday, October 1, 2008, 10:16 AM Are there any opinions as to using histoclear vs. xylene? Thanks! Sarah _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
