Hi Gudrun, Is your friend soaking blocks in ice water before cutting? This is common in animal histology and over soaking before cutting will cause swelling. Different tissue types will swell more quickly than others, but over soaking can eventually affect all tissue.
Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 [EMAIL PROTECTED] -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Wednesday, October 01, 2008 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue swelling out of block Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet