When I was in Prince George, BC we used to tell our satellite labs to dilute
the NBF 20% with ethanol . This worked fine for us and below -20C. At
really cold temperatures it turned slushy rather than solid, but we rarely
got ice crystal holes.
I used to live and work in Winnipeg. I was the section head in histology at
the HSC from 67-74. How are things there, now?
Bryan Llewellyn
----- Original Message -----
From: "Sate Hamza" <[EMAIL PROTECTED]>
To: <[email protected]>
Sent: Thursday, October 02, 2008 7:58 AM
Subject: [Histonet] Winterize formalin?
Dear Histonetters,
A colleague from Boston posted this inquiry to our dermatopathology
mailing
list: "Does anyone [have] the formula to winterize formalin? We were
adding
15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work but
is
not working now at -20" ..
Interestingly, I found out that we do not "winterize" formalin up here in
Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am
wondering
if anyone on the list "winterizes" formalin .. I found the following
abstract of a Japanese paper by searching in Pubmed
http://www.ncbi.nlm.nih.gov/pubmed/3692431
Thank you ..
Sate
--
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada
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