Amos: Isopropyl alcohol (IPA) penetrates less quickly than ethanol, but dehydrates more gently and hardens the tissues less. When the tissue ihas been dehydrated with IPA, you can mixe it in a proportion of 5:1 and 2:1 with mineral oil at 50ºC and by doing so you can ELIMINATE
--- On Fri, 10/3/08, Amos Brooks <[EMAIL PROTECTED]> wrote: From: Amos Brooks <[EMAIL PROTECTED]> Subject: [Histonet] Isopropyl vs Ethanol To: "[email protected]" <[email protected]> Date: Friday, October 3, 2008, 8:31 AM Hi, Is there anyone who uses exclusively isopropyl alcohol to process with rather than ethanol? I have had great results with isopropyl alcohol for some tissues, but I wanted to know if there are any pitfalls to using it exclusively for processing. I've noticed that isopropyl is much cheaper than ethanol, so it leads me to wonder why ethanol is so prevalant for dehydration. Thanks, Amos Brooks _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
