Can you post a pic of this non specific staining? What organ is it?
Is it inside blood vessels?
At 5:27 PM -0500 10/7/08, Jan Shivers wrote:
Addendum... I should have included this information in my original posting...
I'm already doing a 3% H2O2 peroxidase quench (10-15 minutes) and a
10% normal serum protein block prior to primary Ab, and a 2-4% case
species serum addition to the link Ab.
Is there something ELSE that I could use to block binding to
platelets (or what appears to be platelets, though it could be bits
of proteinaceous cellular debris that's binding Ab nonspecifically)?
Jan S
----- Original Message -----
From: Jan Shivers
To: histonet
Sent: Tuesday, October 07, 2008 3:14 PM
Subject: platelets and IHC
Hi all,
Has anyone ever encountered platelets staining nonspecifically when
using an antibody directed against other antigens? If so, how do
you quench that activity? Thanks.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
[EMAIL PROTECTED]
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
