This is our current protocol (on Dako Autostainer):
1.
Pretreat slides in Trilogy (Cell Marque) in pressure cooker for 20
minutes.
2.
Cool in hot soln for 10 minutes.
3.
Rinse in DI H2O.
4.
Peroxidase block for 5 min.
5.
Protein block for 5 min.
6.
SV-40 primary (Chemicon 1:10,000) for 30 min @ RT
7.
Dako Envision Dual link polymer for 30 min @ RT
8.
DAB 5 min.
9.
Counterstain, dehydrate, clear and coverslip.
Hope this helps.
Ashley Troutman BS, HT(ASCP)QIHC
Histopathology Laboratory
Department of Pathology
Vanderbilt University Medical Center
Nashville, TN
<http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/>
<http://www.mc.vanderbilt.edu/> <http://www.vanderbilthealth.com/main/>
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