True, but unless you have every slide on the run as titration the machine wont start. That's a good trick if you just have a titration run.
Mark On Tue, Oct 14, 2008 at 11:37 AM, Sebree Linda A. <[EMAIL PROTECTED]>wrote: > Yes, you can always run in "manual" mode....editing the protocol to > "titration" rather than "antibody". That does indeed allow you to > manually apply the antibody when the instrument signals its time to do > so. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > -----Original Message----- > From: [EMAIL PROTECTED] > [mailto:[EMAIL PROTECTED] On Behalf Of Milne, > Katy > Sent: Tuesday, October 14, 2008 12:45 PM > To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Expiration dates > > Hi Amy, > > I use the Ventana Discovery and I add my antibodies manually as I'm in > research and don't feel like shelling out extra for empty containers to > put my Abs in. Do you have an option on the Benchmark to switch the > primary Ab step to "titration" or something like that (can't remember > what the exact step name is but not the autodispense one). I don't even > know if that's an option on the Benchmark but with the Discovery, I can > just open that machine and add it straight at that step. Perhaps you > could get your antibody out of the dispensor and try it that way. > > Katy Milne > Deeley Research Centre > > - > Message: 4 > Date: Mon, 13 Oct 2008 07:04:10 -0400 > From: "Senn, Amy R" <[EMAIL PROTECTED]> > Subject: [Histonet] Expiration dates > To: <Histonet@lists.utsouthwestern.edu> > Message-ID: > <[EMAIL PROTECTED]> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > > > My supervisor wanted me to ask everyone who's posting about the > expiration dates of antibodies: How can you use expired antibodies on a > stainer such as a Benchmark-which will not allow you to start the run > due to expiration dates on the containers? > > > > > > Thanks!! > > > > Amy Senn > > Camp Hill, PA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet