I'm working with a client who it is growing cells on a demineralized bone matrix. We have processed the tissue both manually and on a VIP and we are seeing that the cells are not attached to the bone and that they are separated away from the bone. We are trying to figure out if this is a processing artifact and what we can do to prevent it. The cells appear to follow the structure of the bone its just that they are separated away from the bone and should be (in a perfect world) attached to or laying on top of the edge of the bone. If someone has worked with these types of samples before and has advice that would be great. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 [EMAIL PROTECTED] www.premierlab.com <http://www.premierlab.com/> Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet