Dear Histonetters, You may recall my earlier post on rings of pig aorta. I have managed to keep them alive quite nicely with DMEM many thanks for the tips.
Unfortunately, I am still struggling to stain for endothelial adhesion molecules. I have read and received various different opinions on fixation after I have made my cryostat sections. Should the slides be fixed immediately, or air dried first? Acetone or formalin? Any tips would be gratefully received. Thank You, Alistair _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet