Hello,
I have been using Gomori`s Aldehyde Fuchsin to stain human skin for elastin. The stain works fine and is clear and specific. I am using light green as a counterstain. My problem is that once stained, the slides have an excess granular purple "dotted" appearance upon the entire surface of the slide. This is not limited to the tissue position upon the slide, but across the clear glass too. We have tried using both superfrost plus charged slides and uncharged slides but the problem still is present. This precipitate is spoiling an otherwise great stain. Does anyone have any idea about what this is and how it could be prevented? (Our next guess would be to re-filter the fuchsin solution prior to use every time) Many thanks Adam Adam Boanas Histology Manager Renovo Ltd PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR RECORDS: >From Monday 07/06/2008 our Telephone number will be as follows: Switchboard: 0161 276 7100 extension 7716 LEGAL NOTICE This e-mail is from Renovo Group plc (Registered No 5427608 England). The information contained in this e-mail and any files transmitted with it are confidential, may be privileged and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient any disclosure, copying, retransmission, distribution, dissemination, action taken or omitted to be taken in reliance upon this e-mail is strictly prohibited and may be unlawful. If you have received this communication in error please notify the sender by e-mail or by telephone on +44(0)161 276 7100, delete it from your system and destroy any copies of it. Please note that e-mails from Renovo Group plc pass through a virus checker but e-mails can be intercepted or affected by different viruses. Neither Renovo Group plc nor the sender accepts any liability or responsibility for viruses or other material introduced with this message and the recipient should ensure that the e-mail and attachments (if any) are actually virus free. Any views expressed by an individual within this e-mail do not necessarily represent the views of Renovo Group plc. No contract may be concluded on behalf of Renovo Group plc by means of e-mail communications. Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester, M13 9XX, UK Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200 Website: http://www.renovo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet