You might haveto make some in-house. The following is easy to make and can be used soon after preparing. Use a good control (skin, appendix) to control your times:
Debidin's Haematoxylin This solution uses sodium hypochlorite as the oxidant in the preparation of alum haematoxylin. This reagent also has the advantage of being a bacteriostat (Debidin 1991). Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5µm. Reagents: Haematoxylin (CI 75290) 2.5g Potassium Alum 50g Distilled water 500ml 5.6% Sodium hypochlorite 2ml Glacial Acetic Acid 2.5ml Dissolve the Alum in distilled water with the aid of heat. Add the haematoxylin and mix. Cover and leave mixture at 60oC overnight. Add the sodium hypochlorite and allow to stand for one week, add acetic acid. Debidin (1991) Histologic 11(2):232. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jo-Ann Bader, Ms. Sent: Tuesday, 21 October 2008 12:07 AM To: Histonet@lists.utsouthwestern.edu Cc: Melina Narlis, Ms Subject: [Histonet] Acidified Hematoxylin Good Morning Everyone, We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: [EMAIL PROTECTED] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
