Molecular Probes (now Invitrogen) makes some really good kits for doing fluorescent labeling of human antibodies that can be applied to human tissue. They also make kits for mouse on mouse applications. These Zenon kits come in a variety of fluorphores and I believe they also have some haptens like biotin that can be used for chromagenic staining. It is essentially the same concept as the Dako Animal Research Kit: pre-labeling the primary mAb with a labeled Fab fragment secondary followed by an Ig block to absorb the unbound secondary antibody.
Scott Turner Scientist II Schering-Plough Biopharma Palo Alto, CA -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of BJON (Brian Johnson) Sent: Friday, October 24, 2008 09:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Human anti Human Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet