----- Original Message -----
From: "Bryan Llewellyn" <[EMAIL PROTECTED]>
To: "McKnight, Tanisha" <[EMAIL PROTECTED]>
Sent: Friday, October 24, 2008 9:34 AM
Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Try porcine pancreatic alpha amylase from Sigma (A 3176). I used the
amount to cover a dime 1 mm deep in 15 mL distilled water and it always
worked for me in an hour at room temperature.
However, in general, you should base your concentration on the amount of
enzyme you need rather than the powder that contains the enzyme. That is
0.5 grams of a 10 units per gram powder is not the same concentration as
0.5 grams of a 100 units per gram powder.
Bryan Llewellyn
----- Original Message -----
From: "McKnight, Tanisha" <[EMAIL PROTECTED]>
To: <histonet@lists.utsouthwestern.edu>
Sent: Friday, October 24, 2008 8:26 AM
Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working
Hi All:
I am in the process of working up a PAS Staining protocol. The PAS stain
is working fine, but I am unable to get my tissue to digest. I am using
.5g of Diastase in 50ml of DI water. I tried heating the solution in a
water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
as the one without digestion. Could I be using the wrong Diastase? If
so, does anyone have any suggestions for a new product?
Thanks,
Tanisha Neely, HT(ASCP)
AP-Histology/Specimen Management
<mailto:[EMAIL PROTECTED]>
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