Hello everyone This is Girish working as toxicologic pathologist for a drug discovery firm based in India. We are in the process of evaluating mitotic indices/figures in the liver sections of rat. Can anyone help regarding what kind of simple histotechniques are useful for this ? When we went through the literature, we could notice that Toluidine blue and Crystal violet staining will serve the purpose to differentiate from apoptotic bodies and pyknotic nuclei. However, we didn't get the staining protocol for either. If anybody has working experience on this, please share your views Regards and thanks Dr Girish B.Chandrashekar Scientist IB Preclinical Safety Evaluation Discovery Research Dr Reddy's Laboratories Hyderabad, India. Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet