Cindi Robinson asks: >>We are currently using 10% formalin fixation on our bone marrow cores. We >>fix for 2 hours minimum prior to decal. We are using Immunocal from Decal >>Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still >>crunchy upon sectioning and we are doing surface decal for up to 30 min. Our >>paths want cores turned out within 24 hrs following procedure. Any >>suggestions?<<
An ordinary decalcifier (such as Decal Corp's Decal, or several others - just don't use nitric acid) should suffice, and will decalcify marrow cores in an hour or two. Bone marrow specimens obtained after 2 PM should be processed the following day. Would your pathologists and oncologists consent to this compromise? - This is a matter on which, in my experience, pathologists and oncologists don't communicate with each other very well. Bob Richmond Samurai Pathologist Knoxville TN In "commUnIcate", the "U" comes before the "I". _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
