I speed up the fixation and decal time by using a platform shaker for both, it enhances the exchange, especially for decal, I would not skimp on fixation before decal, the fixation will protect the tissue from decal and processing, if you do not adequately fix, you can lose the proteins of interest during processing, and that is lose completely so that no retrieval process will work to bring them back.
Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 [EMAIL PROTECTED] www.ihctech.net www.ihcrg.org -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Troutman, Kenneth A Sent: Friday, October 31, 2008 12:18 PM To: Histonet Subject: [Histonet] (no subject) Try the following decal from Surgipath. We used it with great success when I was in another lab. It is a 2 step decal procedure and it can significantly cut down on the total time. http://www.surgipath.com/region/us/product.aspx?p=77 Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN <http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/> <http://www.mc.vanderbilt.edu/> <http://www.vanderbilthealth.com/main/> -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cynthia Robinson Sent: Thursday, October 30, 2008 11:44 AM To: histonet Subject: [Histonet] bone marrow biopsies We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi <http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/> <http://www.mc.vanderbilt.edu/> <http://www.vanderbilthealth.com/main/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet