Piero, We use C4d from American Research Products, Inc., cat. #: 12-50000 at 1:50. On the BenchMark XT we use mild CC1 for 32" at 42 degrees C with AB block.
Hope this helps. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Piero Nelva Sent: Friday, November 07, 2008 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD4 on Ventana Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
