Hello Histonetters, When I compare my MMA bone histology with with paraffin bone sections I always notice the same artifact in the paraffin sections: The bone lining cells seem to fall off the surface, I also noticed osteoclasts here and there that were stripped off. Bone morphology is good (I used EDTA for decal). I suspect that maybe the dehydration was too fast for bone (I used the same protocol as for normal tissue specimens).
What are your experiences with decalcified bone sections? Do you use a different protocol for your VIP? Or are these artifacts inevitable? Thanks, Alexandra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet