Hi Ian - I just happen to have a copy of "Fixation of Fish Tissue" by Fournie,
Krol, Hawkins- CH 34, Academic Press, 2000. I have the excerpt so I do not know the title
of the book.
I quote them- " The osmolality range for mammalian tissues are routinely used.
For fish, glutaraldehyde fixatives in cacodylate buffer wihch fall with in the
osmolality range for mammalian tissues are routinely used. Karnovsky's fixative
(about 2000 mosm) also is often used for marine specimens. The additon of
non-electroylytes (sucrose, glucose, dextran) and electroylytes (CaCL2 and NaCl)
stabilize osmolarity and decrease cell swelling.
Recommended formula:
2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer with 0.6 M sucrose
O.2M sodium cacoduylate buffer, pH7.4 .........250.0 ml
glutaraldehyde, 25% ........................... 50.0 ml
sucrose.........................................100 gm
distilled water .............................500 gm
Hope this is helpful. I have good friends who do a lot of fish histology at
Gulf Coast Research Laboratory, Univ. of Southern Miss. Ocean Springs, MS.
If you have further questions- contact Nancy-Brown Peterson there.
Judy
On Thu, 13 Nov 2008, Ian Montgomery wrote:
What is the recommended EM buffer for sea water fish? I had
thought of using sea water itself but would cacodylate or one of the
phosphates be better and at what molarity?
Ian.
Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
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Judith Williams, PhD, HT(ASCP)
Research Scientist
Department of Comparative Medicine
University of Washington
Seattle, WA 98195
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