Toni, They are fixed for 10 minutes in the fomalin fumes. We do not perform IHC on bone marrow smears, sorry
Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: Rathborne, Toni [mailto:[EMAIL PROTECTED] Sent: Friday, November 14, 2008 1:30 PM To: Joyce Cline Subject: RE: [Histonet] Bone Marrow smears fixation Joyce, How long do you let the slides stay with the fumes? Have you run IHC on slides fixed this way? Thanks, Toni -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of Joyce Cline Sent: Friday, November 14, 2008 12:40 PM To: Histonet Subject: RE: [Histonet] Bone Marrow smears fixation We give our Hematology department Superfrost+ slides to use for smears, because we had the same problem. We also went back to the old fashioned way of fixing the smears. We use a coplin jar with a small piece of papertowel in the bottom and place a few drops of formalin in the coplin jar. The smears are fixed in formalin fumes. We had a lot of smears that were not staining for iron before using the methanol method. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Georgine Whitman Sent: Friday, November 14, 2008 9:51 AM To: [EMAIL PROTECTED] Subject: [Histonet] Bone Marrow smears fixation we are trying to do an iron stain on our bone marrow smears on the Ventana Nexus. We are having trouble with the specimen staying on the slide. I personally have not done iron on smears in years. Our hematology department has been doing them by hand and they are fixed in methanol alcohol. Please Help Thank You Georgine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet