Sounds to me that your having a de-paraffining problem. Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be. Are you on a daily rotation/change of staining/de-paraffin reagents? Maybe, your oven isn't hot enough to melt some paraffin off the slides before de-par!
Josie Britton HT -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Wednesday, November 19, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Uneven H&E Staining Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -----Ursprüngliche Nachricht----- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008 02:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Uneven H&E Staining Recently we have had an issue with uneven H&E staining. Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section. All cut by the same person and the stains were great. What could be the cause of this? Also with the quality of formalin. Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts? What is the cause? Karla Arrington, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
