Great question... I'll have to try this. My immediate thought is "does the hematoxylin interfere?" I'll run a slide this week and let you know... Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN <http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/> <http://www.mc.vanderbilt.edu/> Message: 23 Date: Sat, 22 Nov 2008 15:12:27 +0100 From: "Gudrun Lang" <[EMAIL PROTECTED]> Subject: [Histonet] ISH question To: <histonet@lists.utsouthwestern.edu> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii"
Hi listmembers, In our lab the unstained IHC-slides are thrown away. Now one slide of each case should be stored in an adequate manner to perform an insitu hybridization if required in the future. I want to store it unstained but deparaffinized and coverslipped. So that it can be filed with the other HE-slides and easily found again. We don't want to store them airsealed in the freezer, and IHC is no issue. Has anybody performed an in situ hybridization on previously coverslipped or even stained tissue? I think, that the DNA would be well preserved. What could be a cause of DNA-degradation in this conservation-manner? Thanks for sharing your experiences. Gudrun Lang Histolab, Akh Linz, Austria <http://www.vanderbilthealth.com/main/> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet