Lee and Peggy- We section these eyes all the time with the plastic in place.
I would certainly suggest a gluaraldehyde fixative. Straight NBF is a very poor fixative for the many tissues found in eyes, especially the retina. If you contact me directly, we can talk off line. Eyes have held a special fascination with me for many years. Joe Saby NAMSA ________________________________ From: Lee & Peggy Wenk <[EMAIL PROTECTED]> To: [email protected] Sent: Monday, November 24, 2008 12:47:24 PM Subject: [Histonet] Dissolve plastic I Need Histonetters help. We have an eye with a plastic part for the lens, which is a type of telescope, I've been told. According to the new pathologist, we need to dissolve out this plastic lens, then he can gross the eye and we can process it into paraffin. We've never had to dissolve plastic before. The eye is in 10% NBF right now. I'm guess maybe something like chloroform? But what percent? For how long? Before or after grossing? Has anyone done this before, that can send a procedure to me? Thanks. *************************************** Peggy A. Wenk, HTL(ASCP)SLS Program Director, Schools of Histotechnology Supervisor, Mortuary Services Laboratory Safety Officer, Disaster Preparedness and Quality Assurance Coordinator Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9079 Fax 248/898-9054 E-mail [EMAIL PROTECTED] Web Page: http://www.beaumont.edu/alliedhealth _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
