We make up the hydroquinone reducing solution just before use, but found for the Steiner, since it makes up 60 mL, that if we use half and store the other half in the refrig, we can use it the next day. After that, it doesn't work very well.
As for silver solutions, if you are talking about a certain percent aqueous silver nitrate (say 1% aq. Silver nitrate), we found aq. Silver nitrate is good in the refrig for about 6 months, so we only make up enough to last 2-3 months, to be on the safe side. If you are talking about the silver made up in a buffer solution, then I don't know how long it is good for. If you don't do W-S very often, it just might be best if you make both of these solutions up when the stain is requested. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of V. Neubert Sent: Tuesday, November 25, 2008 2:49 AM To: [email protected] Subject: [Histonet] Warthin-Starry // "Best before" dates Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can keep the AgNO_3 solutions and the hydrochinone solution whitout getting them to "expire". Is there anyone who already had to deal with that question? Regards, Valentin _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
