We take two pieces of fresh tissue (umbilical cord works well) to micro and have them grow positive and negative on individual pieces. After a few days we fix the tissue and embed them together. Works great. PS we do the same when we need fungus controls.
-----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa Sent: Tuesday, November 25, 2008 3:40 PM To: histonet@lists.utsouthwestern.edu; Fischer, R. B Cc: Delcambre, Linda V Subject: Re: [Histonet] gram stain controls I always used actual tissues with gram +/- bacteria because it is not the same (time wise) to stain a histogel than a piece of tissue. Appendix is a good control. René J. --- On Tue, 11/25/08, Fischer, R. B <[EMAIL PROTECTED]> wrote: From: Fischer, R. B <[EMAIL PROTECTED]> Subject: [Histonet] gram stain controls To: histonet@lists.utsouthwestern.edu Cc: "Delcambre, Linda V" <[EMAIL PROTECTED]> Date: Tuesday, November 25, 2008, 3:29 PM My pathologist has made a gram neg/pos control block for us to use. He made this by taking separate cultures of each and suspended them in histo gel. The control stains well. My question is: Since we are performing gram stain on tissues exclusively, will this cultured control be an accepted method as a control for tissues, or must we use tissue with gram pos/neg organisms. Thanks for your reply. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
