In my experience, CD20 does not work in canine or feline tissues. Try CD79a instead. I can't comment on the use of touch preps. With FFPE, Dako TRS pH9 for 20 minutes works great.
-----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sandra Cheasty Sent: Monday, December 01, 2008 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] T Cell and B Cell Markers on Lymph Node Touch Preps Hello, Does anyone have experience with performing Immunocytochemistry (CD3, CD20) on touch preps of canine and feline lymph nodes, particularly the following: Fixation: What reagent(s), what temperature, how long, immediately or wait until air dried, storage post fixing Antigen Retrieval: If formalin is part of the fixative, is there a general rule for HIER on ICC specimens that relates to HIER on paraffin sections of lymph nodes for CD3 and CD20? Thank you so very much. Sandra Cheasty Histology & Necrcopsy Supervisor UW-Madison School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
