I changed over to the Surgipath H&E "system" and after working out timing and water issues, I resolved the "blue haze" problem by increasing the time in the DEFINE to 45 seconds and I have no blue haze. You might have to tinker with the DEFINE and BLUE BUFFER times to optimize the stain. All in all - an excellent, clear, sharp, pathologist-pleasing stain. And - as we all know - "a happy pathologist is a wondrous thing"!
Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet