Hello All,

I have prepared Costar Transwell membrane inserts according to the Corning procedure and I can not keep the little suckers on the slides. Subbed, charged, and celloidin is not working well. With celloidin, they will stay until the last alcohols and then fall off. Any suggestions? I have left the slides in a 60oC oven all night before staining.

I seemed to have become a specialty histo lab. I would really appreciate someone else figuring out what to charge but it's left to me. Is there a lab out there that has a great price list I can "borrow"? As a for instance, I have spent 3 days working on these darn membranes with nothing to show yet and have no idea what to charge. I do lots of hand processing also. What's fair for that?

Thanks for all the help!! (Corning hasn't gotten back to me yet.)
Happy Friday!!
Mary Lou

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