Try this processing schudule for mouse tissue: 70% 15 min 95% 15 min 95% 15 min 100% 15 min 100% 15 min 100% 15 min 100% 15 min Xylene 15 min Xylene 15 min Paraffin 15 min Paraffin 15 min Paraffin 15 min Paraffin 15 min Good luck! Susan Bincsik Does anybody have a protocol for this? My last batch of these came out VERY dry and crunchy when run with other tissues on my standard protocol, which is as follows: (They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before processing.)
70%: 30 min 80%: 30 min 95%: 45 min 95%: 45 min 100%: 45 min 100%: 45 min xylene: 45 min xylene: 45 min Paraffin: 30 min Paraffin: 30min Paraffin: 30 min My other thought is that something is up with our VIP 5 processor, though no error messages are showing up. Any and all suggestions are most welcome. Thanks in advance, Kathleen Roberts Rutgers University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
