Andrea,
We stain for murine dendritic cells very frequently. CD11c (HL3 clone) is
excellent from BD Biosciences using fresh snap frozen tissue with spleen as
the positive control. Fixation is with our beloved 25% ethanol/75% acetone
for 5 min at RT then going directly to buffer from fixative. The sections
are air dried overnight before fixation. Dec 205, NLDC 145 from Serotec
works but we have superior results with the BD Pharm rat antiMouse CD11c HL3
clone.
There are a whole series of new DC antibodies out there, used by our FACS
tech, but I have not tried them on frozen sections yet. I would still used
the same tissue preparation and fixation if we did try them but also toss in
cold acetone just for posteriety sake. The CD11c we work with is
biotinylated, which simplifies everything since we come back with
Streptavidin Alexa dyes for single and double immunofluorescence work these
days. It is an Armenian hamster host IgG1.
Happy Holidays to you
Gayle Callis
HTL(ASCP)HT,MT
Bozeman MT
----- Original Message -----
From: "Andrea Hooper" <anh2...@med.cornell.edu>
To: "Histonet" <histonet@lists.utsouthwestern.edu>
Sent: Friday, December 12, 2008 3:11 PM
Subject: [Histonet] mouse dendritic cells
Is anyone staining for mouse dendritic cells either in paraffin or frozen
sections? If so, what markers/antibodies are you using?
Thanks, ANDREA
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