We actually do that. We are lazy though, and use the water bath by our microtome(approx 43 F). After cutting is done, of course. That way you don't have anything extra to clean up. (I HOPE everyone cleans their microtome waterbaths daily!) :) We sometimes have to use the waterbath in the fume hood. Keep in mind that the air movement will lower the temp of the bath (especially during Wisconsin winters!). We usually put a small metal tray over the bath leaving an opening just large enough for the coplin jar and monitor the water temp continuously. Claire
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 12/12/2008 3:09 PM To: HISTONET ...; TIM MALLOY; TIMOTHY MALLOY Subject: Re: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS I used the MW oven to heat-boost the slutions but the actual staining was done in a water bath. Why don't you try a water bath at 60ºC? rené J. --- On Fri, 12/12/08, TIMOTHY MALLOY <tmallo...@hotmail.com> wrote: From: TIMOTHY MALLOY <tmallo...@hotmail.com> Subject: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS To: "HISTONET ..." <histonet@lists.utsouthwestern.edu>, "TIM MALLOY" <tmal...@cox.net> Date: Friday, December 12, 2008, 4:52 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
