Black Pigment? Paint "dust" from a slide etcher? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aaron Rose Sent: Tuesday, December 16, 2008 5:26 PM To: Tony Henwood; Michele Wich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] at a troubleshooting loss
This may be a silly suggestion but Have you been filtering your hematoxylin? I have seen the sediments that form over time. I'm not sure if this could be the dark regions you're seeing but I figured I'd throw it out there. -Aaron -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, December 16, 2008 3:18 PM To: Michele Wich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] at a troubleshooting loss ?carbon from lead pensils used for labelling slides. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Wednesday, 17 December 2008 4:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] at a troubleshooting loss A couple questions for those who are troubleshooting savvy: Other than inadequate fixation, water being introduced during processing, or too much heat, what can cause fuzzy, uneven hematoxylin staining with poor nuclear detail? And secondly, aside from formalin, are there any other common agents of blackish pigment in H & E staining? (The artifact appears on the slides both on and around the tissues.) Any feedback is GREATLY appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet