Hi- I am trying to find a suitable Myelin stain for LCM that maintains RNA integrity. I am currently testing Luxol Fast Blue, Oil Red O and Fluoromyelin but none seem to keep the RNA in tact all that well (although I am still trying to modify these procedures). My ultimate goal is to dissect regions of white matter where demyelination has occurred due to disease. Anyone have any other suggestions?
Also, does anyone know of any Oil Red O procedures that do not require formalin fixation of the tissue? I have tried fixing the tissue in isopropanol with poor histological results. Any suggestions? Thanks and happy holidays! -Nicole _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
