Hi dear listmembers, Is it generally accepted, that formaldehyd fixation in an acid environment is less effective than in basic? Or in other words: Does formaldehyde bind to proteins in acid solution to a smaller amount than in basic solutions?
I have found a publication (Theis, 1944) where the the amount of bound formaldehyde was plotted against pH. And the line increased with the pH. A few years later Gustavson published that Theis' methods of measurement were wrong and also his findings about the reactionpartners of formaldehyde. But I cannot find a newer paper about the effect of pH on formaldehydfixation. Can anyone help me out? Froehliche Weihnachten! Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
