Use Ulex europaeus lectins. René J. --- On Wed, 12/24/08, TF <ti...@foxmail.com> wrote:
From: TF <ti...@foxmail.com> Subject: Re: Re: [Histonet] Capillary counting To: "rjbuesa" <rjbu...@yahoo.com>, "histonet" <histonet@lists.utsouthwestern.edu>, "Nejat Yilmaz" <nyil...@mersin.edu.tr> Date: Wednesday, December 24, 2008, 1:48 PM _filtered #yiv1672193670 { font-family:宋体; } _filtered #yiv1672193670 { font-family:Verdana; } _filtered #yiv1672193670 { } _filtered #yiv1672193670 {margin:72.0pt 90.0pt 72.0pt 90.0pt;} #yiv1672193670 P.MsoNormal { TEXT-JUSTIFY:inter-ideograph;FONT-SIZE:10.5pt;MARGIN:0cm 0cm 0pt;FONT-FAMILY:"Times New Roman";TEXT-ALIGN:justify;} #yiv1672193670 LI.MsoNormal { TEXT-JUSTIFY:inter-ideograph;FONT-SIZE:10.5pt;MARGIN:0cm 0cm 0pt;FONT-FAMILY:"Times New Roman";TEXT-ALIGN:justify;} #yiv1672193670 DIV.MsoNormal { TEXT-JUSTIFY:inter-ideograph;FONT-SIZE:10.5pt;MARGIN:0cm 0cm 0pt;FONT-FAMILY:"Times New Roman";TEXT-ALIGN:justify;} #yiv1672193670 A:link { COLOR:blue;TEXT-DECORATION:underline;} #yiv1672193670 SPAN.MsoHyperlink { COLOR:blue;TEXT-DECORATION:underline;} #yiv1672193670 A:visited { COLOR:purple;TEXT-DECORATION:underline;} #yiv1672193670 SPAN.MsoHyperlinkFollowed { COLOR:purple;TEXT-DECORATION:underline;} #yiv1672193670 SPAN.EmailStyle17 { FONT-WEIGHT:normal;COLOR:windowtext;FONT-STYLE:normal;FONT-FAMILY:Verdana;TEXT-DECORATION:none;} #yiv1672193670 DIV.Section1 { } #yiv1672193670 UNKNOWN { FONT-SIZE:10pt;} #yiv1672193670 BLOCKQUOTE { MARGIN-TOP:0px;MARGIN-BOTTOM:0px;MARGIN-LEFT:2em;} #yiv1672193670 OL { MARGIN-TOP:0px;MARGIN-BOTTOM:0px;} #yiv1672193670 UL { MARGIN-TOP:0px;MARGIN-BOTTOM:0px;} So any one can provide some cell makers for staining of blood vessels? I only know CD31. 2008-12-25 TF 发件人: Rene J Buesa 发送时间: 2008-12-19 04:53:43 收件人: histonet; Nejat Yilmaz 抄送: 主题: Re: [Histonet] Capillary counting Nejat: There are several stains for capillaries: 1- Campbell and Alexander (tested by Mallory,1938): black agsinst colorless bbackground. 2- Doherty, Suk and Alexander, 1938: differential staining of blood in capillaries. 3- Pickworth, 1934: blood vessels (capillaries) in thick sections of brain. 4- Slominski and Cunge (tested by Romeis, 1948): differential staining of capillaries, and 5- Ziegler 1945 (tested by Riley, 1945): demonstration of capillaries in wholemounts of cornea. Although developed for other tissues it is very likely that they will work on skeletal muscle. Ren?J. --- On Thu, 12/18/08, Nejat Yilmaz <nyil...@mersin.edu.tr> wrote: From: Nejat Yilmaz <nyil...@mersin.edu.tr> Subject: [Histonet] Capillary counting To: histo...@lists.utsouthwestern.edu Date: Thursday, December 18, 2008, 2:26 PM Hi All, We need a protocol demonstrating capillaries in sceletal muscle tissue other than immunohistochemistry. We're planning to compare number of capillaries between different experiment groups for evaluating angiogenesis. Is there any special stain to suggest for this purpose? Thanks in advance. Necat Yilmaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet