You can possibly use it especially if it has been used for IF. What you need to do is to get a slide that has a known positive for the antibody, try the dilution for WB with your standard protocol and see if it works. If is too light or too dark you will have to titer it (which should be done in the first place). If there is no staining you may have to try different things like enzymatic antigen retrieval or heat etc. In most cases you can get it to work to some degree but it is better if you get one that has been used with tissue. The first thing I do is call the company and talk to the technical personnel to see if they have tested it on tissue samples. It takes some work.
Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, January 02, 2009 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Differences between WB antibody and IHC antibody If anyone replies with posting to the list, please forward it. I've always wondered the same thing. On Fri, Jan 2, 2009 at 8:15 AM, TF <ti...@foxmail.com> wrote: > Hi > Just wondering whether the antibody for WB (only WB, IP on datasheet) can > be used for IHC use? > If not, why? > > Can I just increase the concentration for IHC application? > > I want to learn more about the underlying production processes. > Thanks! > > > 2009-01-02 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- At the end of what is called the "sexual life" the only love which has lasted is the love which has everything, every disappointment, every failure and every betrayal, which has accepted even the sad fact that in the end there is no desire so deep as the simple desire for companionship. -Graham Greene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
