I have the same post last week but did not get reply.
Actually I have a IgM primary antibody and I can just visualize the staining 
with IgG 2nd antibody.
SOme IgG 2nd antibody can just applied to do so. This has been proved. But you 
should test yours anyway.


2009-01-03 



TF 



发件人: anjan kumar 
发送时间: 2009-01-03  00:33:39 
收件人: triple immunohistochem 
抄送: 
主题: [Histonet] IgM monoclonal antibody compatibility 
 
hi, 
  i wanted to  know if there are any difference in selecting secondary antibody 
against IgM compared to secondary antibody for IgG.
as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary 
antibody should i select, currently i m hoping that goat anti mouse will work.
can any one plz suggest me on this.
Regards,
Dr. Anjan Kumar
Junior Research Scientist
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