Another option: When you fix overnight, is that with the colon opened and in fixative, or with the tissue in cassettes in the fixative overnight? That should be fine.
Or is that placing the unfixed colon intact into the fixative overnight? If it is this way, then the fixative has not penetrated to the center of the colon, so there is poorly fixation. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph N. Pastore Sent: Saturday, January 03, 2009 7:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] colon resection fixation On our last HistoQuip it was mentioned that our section for a colon specimen was under fixed or poorly fixed. Now ...we routinely fix in formalin overnite and the overnite in O-Fix. But there are times when the specimen has to be stored in the OR refrig after COB. Can this be the problem and does anyone have ideas as to how this can be avoided? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet