When we process embryos this old, the are so large and their skin is so
thick that we have to make a long slit down their back with a razor
blade to allow infiltration. Otherwise, we get a soft, mushy mess that
can't be sectioned. Depending on what you are interested, you could
them in half down the midline, or do a slit down the back or belly.
Good luck!
--
Jennifer L. Springsteen, B.S.
Lab Manager, Assistant Scientist
Lillehei Heart Institute Histology & Microscopy Core Facility
University of Minnesota School of Medicine, Division of Cardiology
312 Church Street SE
4-266 Nils Hasselmo Hall
Minneapolis, MN 55455
Lab: 612-626-3090
Cell: 651-357-7916
Fax: 612-624-8118
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