4% PFA so u mean I should expose more of the damaged site (it is at the bottom of the brain, or olfactory bulb) and immerse the whole brain in a larger volume of fixatives?
I think PFA penetrate quite fast, and I took all the skins & muscles off the skull before put the who brain with skull into PFA for 1-2 days. It should work... The perfusion is not well in many conditions - in my injury model, part of the blood supply was interrupted. 2009-01-12 TF 发件人： Geoff McAuliffe 发送时间： 2009-01-12 22:59:22 收件人： tifei 抄送： histonet 主题： Re: [Histonet] Fixation of the damaged brain tissue Hi TF: Your problem should not be happening. Sounds like poor perfusion followed by immersing the whole brain (with the wound deep in the interior) in a small volume of fix at 4 degrees C. What fixative are you using? Geoff TF wrote: > Hi, I just want to fix the damaged brain tissue (2-48 hours after I made the > injury). > Because the injury damaged the surrounding blood supply, and after perfusion > I normally got a mass of soft, shapeless tissue surrounding the injury site... > I have also tried post-fixation of 24-48 hours before removing the brain from > in situ. But it does not help a lot. > Can anyone provide some suggestions? > > 2009-01-12 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu **********************************************
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