Bob, So which type of sectioning are you doing now? Cryotomy or vibratome? And how thick do you want the sections to be? If frozen sections on snap frozen mouse brain, simply turning the temperature up to -16C or so will make sectioning much easier and get rid of the shredding, friable sections. Also, are you fixing then sucrose cryoprotecting after NBF or 4% paraformaldehyde fixation of the brain (can be either perfused or immersion fixation)?
Gayle Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
