Hi: We routinely perform immunohistochemistry (IHC) to detect antigens on rat tissues. A mouse mAb should be fine for use in your rat samples. You will not need a special kit, just an anti-mouse secondary Ab. One runs into trouble when they use a mouse (or rat) primary on mouse (or rat) tissue, respectively. Then there are kits (i.e. Mouse-on-Mouse kits) that can decrease background staining due to the anti-mouse IgG secondary you would need to use to detect the primary Ab. From my limited knowledge of IHC, I would say that you could certainly use a biotinylated goat-anti-mouse secondary followed by ABC. I do this all the time and use DAB for visualization of my antigen of interest.
Cheers: ---mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: prit...@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, January 14, 2009 2:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining rat tissue with monoclonal antibody? Good afternoon. I just had someone ask me about staining rat tissue using an monoclonal (mouse) antibody. I have never done this before and so was wondering if there is anything in particular I need to keep in mind. Does mouse antibodies cause a problem when staining rat tissues? Do I have to use a specific kit or specific secondary antibody? Can I use a biotinylated goat-anti-mouse secondary followed by ABC? Thanks for your help, Eva _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet