Use an aldehyde block on the slides
(http://stainsfile.info/StainsFile/stain/schiff/reaction-block.htm) as
glutaraldehyde often has an active aldehyde group ready to react, then try a
PAS using a Schiff's reagent made with acriflavine, proflavine or acridine
orange (http://stainsfile.info/StainsFile/stain/schiff/pafs.htm and
http://stainsfile.info/StainsFile/stain/schiff/pas-standard.htm). After 10
minutes in the Schiff's reagent after step 7, put the section in 1% acid
alcohol for 5 minutes or so, then wash as in step 8 and carry on.
Bryan Llewellyn
----- Original Message -----
From: "Bill McManus" <bill.mcma...@usu.edu>
To: "Paula Pierce" <cont...@excaliburpathology.com>;
<histonet@lists.utsouthwestern.edu>
Sent: Wednesday, January 14, 2009 12:14 PM
Subject: Re: [Histonet] blocking aldehydes for staining
Paula:
Do you know of any stains that fluoresce and stain starches?
On Jan 14, 2009, at 1:11 PM, Paula Pierce wrote:
Congo Red is for amyloid.
I suggest you use an iodine solution to stain starch. It will stain it
black.
From: Bill McManus <bill.mcma...@usu.edu>
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, January 14, 2009 1:51:46 PM
Subject: [Histonet] blocking aldehydes for staining
>
>
> We are trying to use Congo Red to stain starch for confocal, but it
> is staining everything. The sample is condensed milk and very
soft
> without fixation. Does anyone know of a good method to block the
> glutaraldehyde so that the Congo Red will only stain the starch?
>
> Bill McManus
> Western Dairy Center
> Utah State University
> bill.mcma...@usu.edu
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Bill McManus
Western Dairy Center
Utah State University
bill.mcma...@usu.edu
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
