Hi Everyone, Happy Friday! I am looking to use PhosStop and Complete (both from Roche) in my fixative for cryosections (and cytospins). I have used in in the past in formalin for FFPE xenografts, and now I want to use it as a cryo-section fixative. What I want to know is:
has anyone tried this? is this solution re-usable (for more than one rack of slides); will I need a fresh batch for each rack or can I re-use it for several racks during the same day. They are both supposed to be stable, once dissolved, at 4C for 1-month. I don't know how much "depletion" or inactivation will occur if I use this reagent repeated during the day (it would probably only be about 8 racks of slides in the fixative) should I keep it cold (ie - fix the slides @ 4C) or can I use it at Room Temp (fix the slides at RT) Any insight would be great! Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
